How to optimize my MySQL query to use less mysql connection?

Please can you let me know, How to optimize my MySQL query to use less mysql connection? what is good parameter to set for optimize? This is my default my.cnf settings.
[mysqld] local-infile=0 max_connections=800 max_user_connections = 50 datadir="/var/lib/mysql" socket = "/var/lib/mysql/mysql.sock" key_buffer = 256M table_cache = 28560 open_files_limit=50000 join_buffer_size = 2M read_buffer_size = 2M sort_buffer_size = 6M tmp_table_size=128M read_rnd_buffer_size=4M max_heap_table_size=64M myisam_sort_buffer_size=64M thread_cache_size=8 thread_cache=512 query_cache_limit=12M query_cache_size=256M thread_concurrency = 8 wait_timeout=1000 connect_timeout=1000 interactive_timeout=1000 #log-slow-queries = /var/log/mysqlslowqueries.log max_allowed_packet=268435456 default-storage-engine=MyISAM innodb_file_per_table=1 [isamchk] key_buffer =64M sort_buffer_size = 64M read_buffer = 16M write_buffer = 16M [myisamchk] key_buffer = 64M sort_buffer_size = 64M read_buffer = 16M write_buffer = 16M [mysqlhotcopy] interactive-timeout [mysqld_safe] log-error="/var/log/mysqld.log"